![]() ![]() The photograph was taken under 20× objective (10 × 20). The migration ability promoted by siLHPP-pool was partially inhibited by knocking down YTHDF2. (G) Trans-well assay (representative wells were presented). Colony formation rate promoted by siLHPP-pool was partially inhibited by knocking down YTHDF2. (F) Colony formation assay (representative wells were presented). The migration ability promoted by siNKX3–1-pool was partially inhibited by knocking down YTHDF2. (E) Trans-well assay (representative wells were presented). Colony formation rate promoted by siNKX3–1-pool was partially inhibited by knocking down YTHDF2. (D) Colony formation assay (representative wells were presented). (D)-(G) The phenotypes induced by rescue experiments. Knock-down of LHPP partially rescued the expression of pAKT(s473) at protein level reduced by knock-down of YTHDF2. The suppressed expression of LHPP induced by siLHPP-pool was partially restored by knock-down of YTHDF2 (siYTHDF2-pool) at protein level (C) Western blot assay. The suppressed expression of NKX3–1 induced by siNKX3–1-pool was partially restored by knock-down of YTHDF2 (siYTHDF2-pool) at protein level. (A)-(C) The protein level changes of rescue experiments in PC-3 cell line. Rescue experiments between YTHDF2 and LHPP/NKX3–1/pAKT. The Creative Commons Public Domain Dedication waiver ( ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.Īdditional file 10: Figure S8. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. ![]()
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